นโยบายงานวิจัย /จรรยาบรรณนักวิจัย /ระดับคุณภาพบทความวิจัยตีพิมพ์ /ระดับคุณภาพผลงานวิชาการ /แหล่งทุน /ดาวน์โหลด /ฐานข้อมูลวิจัย /วิเคราะห์-สังเคราะห์งานวิจัย /ลิขสิทธิ์ /ข่าว


Optimization of process parameters for phenolics extraction of Cratoxylum formosum ssp. formosum leaves by response surface methodology

Author

- Bancha Yingngam  Nuttapun Supaka
Wandee Rungseevijitprapa


Journal

- J Food Sci Technol

Volume

- 13

Year

- 2013

Publication type

- Research article (Inter)

Page list

- Doi

Abstract

    Leaves of Cratoxylum formosum ssp. formosum
(Cff) contain high amounts of phenolic compounds with
potential anti-oxidant properties. In this study, the effects of
the extraction method on the recovery of phenolic compounds
from Cff leaves were investigated. Response surface methodology
(RSM) was used to optimize the extraction conditions
based on the simultaneous maximization of yields of total
phenolics, chlorogenic acid, and antioxidant activity. The
extraction conditions were investigated using a centralcomposite
rotatable design of response surface methodology
(RSM-CCRD). The independent variables used were ethanol
concentration, extraction temperature, and time. The experimental
results fitted the model well and the investigated
variability was explained. Maximal yields of total phenolics,
chlorogenic acid, and the extract with good antioxidant activity
were achieved by using an ethanol concentration of
38.83 %v/v, temperature of 59.76 °C, and time of 5.50 h.
These conditions gave the extraction yields of total phenolics,
chlorogenic acid, and anti-oxidant activity of 43.52±3.66 mg
gallic acid equivalent (GAE)/g, 119.36±6.34 mg/g, and 0.82±
0.08 mmol trolox equivalents (TE)/g dry powder of plant
leaves. The experimental values agreed with those predicted
within a 95% confidence interval, thus indicating the suitability
of RSM in optimizing the extraction of phenolics from Cff.


Keywords

    Cratoxylum formosum ssp. formosum(Jack) Dyer . Response surfacemethodology . Extraction . Phenolics . Optimization