Influence of elicitors on plumbagin accumulation in Drosera indica whole plant culture was investigated. Treatment of plantlets with 150 mg/l chitosan and 100 μM salicylic acid mostly stimulated plumbagin production after 3 days of elicitation, while 0.5 mg/ml yeast extract and 50 μM methyl jasmonate elicited at 6 days. Yeast extract (0.5 mg/ml) was the most efficient elicitor to enhance plumbagin production (2.69±0.03 mg/g dry wt.) by 5.4-fold over the control plant (0.50±0.01 mg/g dry wt.). Present results indicated the effectiveness of elicitation on plumbagin accumulation in in vitro culture of D. indica.